mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab00345(SNCA antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain using FNab00345(SNCA antibody) at dilution of 1:50
Immunofluorescent analysis of SH-SY5Y cells using FNab00345 (alpha-synuclein antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.